Researchers probe sperm viability in dead stallions
© University of Wisconsin Department of Animal Sciences 
© University of Wisconsin Department of Animal SciencesSperm stored inwards a dead stallion’s epididymis tin rest feasible for upward to 96 hours later on expiry provided it is stored at 4 degrees Celsius, Castilian researchers convey shown.
It is possible to salve sperm from the tail of the epididymis – the long convoluted underground into which the sperm locomote yesteryear later on beingness produced inwards the testicles.
As good every bit acting every bit a reservoir for the sperm, the epididymis provides an surround inwards which they tin mature as well as locomote able to fertilise oocytes.
The physical care for for harvesting as well as freezing the sperm inwards such a mode is non widely available, important access to a suitable facility powerfulness non endure possible.
Research at the Department of Physiology at the University of Murcia inwards Kingdom of Spain suggests sperm stored inwards the epididymis for upward to 96 hours at 4 degrees Celsius tin endure cryopreserved successfully as well as withal retain the powerfulness to fertilise.
In locomote published inwards the journal, Animal Reproduction Science, Luis Vieira as well as others studied the viability of sperm stored inwards the epididymis obtained from castrated horses.
Four unlike veterinarian surgeons provided the scientists amongst a amount of 82 testes amongst attached epididymides from castrated horses.
The testes were transported to the laboratory inwards insulated containers at ambient temperature inside 1 hr of castration.
At the laboratory, the epididymides were washed inwards physiological saline, wrapped inwards foil to foreclose them drying out, as well as stored inwards a fridge at 4º C for upward to 96 hours.
The scientists harvested the sperm yesteryear introducing a syringe as well as needle into the vas deferens as well as flushing out the epididymal contents. This was carried out inwards a sleeping room chilled to 4º C. On average, they recovered 0.7ml of fluid containing 6.5 x 109 spermatozoa/ml from each epididymis.
The sperm were mixed amongst extender to give a concentration of 100×106 cells /ml earlier beingness frozen as well as stored inwards liquid nitrogen.
Measurements of viability of sperm inwards the epididymal fluid were made at diverse stages inwards the procedure.
Viability was practiced (85 percent) if the sperm were harvested on the twenty-four hours of castration, as well as remained at to a greater extent than than lxxx percentage until 72 hours later on castration. Sperm stored for 96 hours earlier harvesting were significantly less viable.
After dilution inwards the freezing media as well as storage at 4º C for xxx minutes, viability remained practiced for sperm harvested inside 48 hours of castration. Viability was lower inwards sperm collected 72 as well as 96 hours later on castration.
On the other hand, fourth dimension (up to 96 hours) from castration to harvesting did non seem to acquit on the viability later on freezing as well as thawing, which was nearly 35 percent.
Other measures of sperm viability as well as tests of their powerfulness to fertilise inwards vitro, were carried out, including chromatin condensation, ROS generation, poly peptide tyrosine phosphorylation as well as heterologous fertilization rate. These showed that epididymal stallion sperm stored for upward to 72 hours inwards the epididymis at 4°C followed yesteryear cryopreservation, maintained both viability as well as powerfulness to fertilize inwards vitro.
Although these were laboratory tests of viability, epididymal sperm convey been used successfully. For example, Stallion AI services inwards the U.K. written report that pregnancy results are beingness achieved, although fertility is probable to endure lower than that of semen collected as well as frozen inwards the traditional manner. They recommend that this physical care for should endure used every bit a concluding resort as well as non every bit a replacement for traditional freezing methods.
Equine spermatozoa stored inwards the epididymis for upward to 96h at 4°C tin endure successfully cryopreserved as well as hold their fertilization capacity.
Vieira LA, Gadea J, García-Vázquez FA, Avilés-López K, Matás C.
Anim Reprod Sci. (2012). S0378-4320(12)00342-9.
doi: 10.1016/j.anireprosci.2012.10.027
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